UNITEFA   23945
UNIDAD DE INVESTIGACION Y DESARROLLO EN TECNOLOGIA FARMACEUTICA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
IMPROVED ANTIBIOFILM ACTIVITY OF CIPROFLOXACIN LOADED IN EUDRAGIT E100 AQUEOUS DISPERSIONS AGAINST FLUOROQUINOLONE-RESISTANT Pseudomonas aeruginosa.
Autor/es:
SMANIA, ANDREA; CORTI, MELISA BELÉN; ALOVERO, FABIANA L.; COLQUE, ANTONELLA
Lugar:
BUENOS AIRES
Reunión:
Congreso; REUNION CONJUNTA DE SOCIEDADES DE BIOCIENCIAS; 2017
Institución organizadora:
SAP, SAFE, SAIC, SAH, SAFIS, SAB, SAA, SAI, SAIB, SAIC
Resumen:
Rising antibiotic resistance and declining development and approvalof new drugs motivates approaches to increase the efficacyof existing antibiotics. Previously we reported that fluoroquinolones-containing Eudragit E100 aqueous dispersions exhibitenhanced bactericidal effect against multidrug-resistant P.aeruginosain planktonic state. However, P.aeruginosa is an opportunisticpathogen with ability to form biofilms, which are inherently resistantto both antimicrobial agents and host defenses, causing seriousproblems in the chemical, medical and pharmaceutical industries.Therefore, there is a significant need to develop alternatives to preventbiofilm formation and, more importantly, to disperse or eradicatepreattached biofilms.The aim of this work was to evaluate the effect of Ciprofloxacin-containing Eudragit E100 aqueous dispersions (EuCl-CIP) onbiofilms of P.aeruginosa formed in Mueller-Hinton broth with standardizedbacterial inoculum (106 CFU/mL) and incubated at 37oCfor 48h. The remaining metabolic activity after 24 h of treatmentexposure was quantified (490 nm) using the tetrazolium salt XTTreduction assay. For comparative purposes, the effect of free-drugpolymer aqueous dispersions (EuCl) and some concentrations ofciprofloxacin (CIP) were evaluated simultaneously.Both EuCl and CIP showed partial reduction of cell viability ina concentration-dependent manner. However, the effect was increasedwhen mature biofilms were treated with EuCl-CIP.The biofilm matrix of P.aeruginosa contains a high proportion ofalginates, among other components. The positive charges presenton Eudragit E100 would be interacting with such components causingalterations in matrix integrity and making CIP more accessibleinto biofilm.Using the Eudragit E100 cationic polymer as carrier for antimicrobialsis an effective strategy to enhance the efficacy of the drugs onbacterial biofilms, in addition to their effect on planktonic cells.