UNITEFA   23945
UNIDAD DE INVESTIGACION Y DESARROLLO EN TECNOLOGIA FARMACEUTICA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
In vivo effect of 5-fluorouracil alone or in combination with albendazole against Echinococcus granulosus.
Autor/es:
BENOIT, JEAN PIERRE; ULLIO GAMBOA GABRIELA; PENSEL, PATRICIA; ELISSONDO, CELINA
Lugar:
Mar del Plata
Reunión:
Congreso; Reunión Conjunta 2016 SAIC, SAI, SAFE; 2016
Institución organizadora:
SAIC, SAI, SAFE.
Resumen:
Cystic echinococcosis CE is a zoonotic disease caused by the larval stage of Echinococcus granulosus. The drugs commonly used against CE are benzimidazoles. Unfortunately, 20-40% of cases do not respond favourably to such chemotherapy. Consequently, the search of new therapeutic alternatives such as the use of anticancer drugs has increased. In previous works, we reported the in vitro effect of 5-fluorouracil (5-FU) combined with albendazole (ABZ) on the larval stage of E granulosus. The combination of 5-FU+ABZ had a stronger effect than that did both drugs alone. The goal of this study was to compare the clinical efficacy of 5-FU alone or combinated with ABZ in mice infected with E. granulosus. The procedures and the protocols involving experimental animals were approved by the Institutional Animal Care and Use Committee (RD 148/15) of the FEyN UNMdP. Female CF-1 mice (n=50)infected with E. granulosus were allocated into the following groups: 1) Unmedicated control group; 2) Saline control group, 3) ABZ group, 4)5-FU group, 5) ABZ+ 5-FU group. ABZ suspension (5 mg/kg) was administered daily during 30 days by oral route. 5-FU (10 mg/kg) was injected in the tail vein weekly for 5 consecutive weeks. All treatments resulted in a statistically significant reduction on the cysts weight compared to those obtained for unmedicated mice (P˃0.05). Co-administration of 5-FU with ABZ did not enhance the in vivo efficacy of drugs alone related to the cyst weight (P˃0.05). However, SEM studies revealed greater damage extension after treatment with 5-FU+ABZ compared to the monotherapy. In conclusion, co-treatment of 5-FU with ABZ improved the in vivo effect of monotherapy only at ultrastructural level. Since 5-FU did not cause toxic effect, further in vivo studies will be performed by adjusting the dosage and frequency of treatment.