UNITEFA   23945
UNIDAD DE INVESTIGACION Y DESARROLLO EN TECNOLOGIA FARMACEUTICA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Characterization of cytotoxic CD8+ T lymphocyte (CTL) effector and memory response induced by a novel formulation strategy for the CPG-ODN adjuvant using a nanostructure platform
Autor/es:
ANA LAURA. CHIODETTI; FERNANDA. SÁNCHEZ VALLECILLO; M. INÉS CRESPO; VIRGINIA. AGUIRRE; SANTIAGO D. PALMA; DANIEL A. ALLEMANDI; M. CRISTINA. PISTORESI-PALENCIA; BELKYS A. MALETTO
Lugar:
Buenos Aires
Reunión:
Congreso; Latin American Society for Immunodeficiencies (LASID), Sociedad Argentina de Inmunologia (SAI), French Society for Immunology (SFI); 2015
Institución organizadora:
Sociedad Argentina de Inmunologia
Resumen:
Background: Previously, we have shown that CpGODN formulated with a nanostructure of 6Oascorbyl palmitate (CoaASC16) has the capacity to enhance a specific humoral and cellular (CTL and Th1) immune response in compare to the CpG ODN solution alone using OVA as an antigen model. In addition, Coa‐ASC16 alone elicited IL‐6 production. Here, we farther characterized the CTL response and the possible signals involved on its development. Methods: To study effector characteristics of CD8+ T lymphocytes, mice were subcutaneously immunized on day 0 with OVA/CpG‐ODN/Coa‐ASC16 or OVA/CpG‐ODN. Seven days after immunization, spleen cell suspensions were cultured with medium or OVA257?264 (1 µg/ml) for intracellular staining. To test an effector memory CTL response, we performed an in vivo cytotoxicity assay 30 days after immunization with OVA/CpG/Coa‐ASC16. To seek if the CTL response was IL‐6 dependent we repeat the assay in Il‐6‐/‐ mice. Results: Mice immunized with OVA/CpG‐ODN/Coa‐ASC16 presented a higher frequency of CD8+ T lymphocytes producing pro‐inflamatory cytokines IFN‐γ (0.53±0.11 vs 0.05±0.02; p