Comparative seroepidemiological surveillance of visceral leishmaniasis and its association with diabetes co-morbidity in Osun State, Nigeria

ATANDA JOY ADEYEMI; FADIYA OLUWADAMILOLA VICTORIA; EBENEZERIDOWUAJAYI; MUSAABIDEMIMUHIBI

Barcelona

Conferencia; IFCC-EFLM 2019 European Congress of Clinical Chemistry and Laboratory Medicine, Barcelona, Spain; 2019

Visceral leishmaniasis is the most severe form of leishmaniasis and the second largest parasitic killer in the world after malaria. This sero-epidemiology surveillance was carried out to ascertain visceral leishmaniasis incidence in Osun State, Southwestern Nigeria. Methods A total of 272 volunteers from different towns of the State were enrolled for participation in this study. They were grouped viz.: Control, Diabetic, Diabetic Foot Ulcer in order to investigate the incidence of VL and the association of the disease co-morbidity with diabetes viz-a-viz implication on biochemical parameters of kidney (Urea and Creatinine) and liver (ALT and AST) functions. Using standard protocols of ELISA, Buffy coat and thin film techniques, VL was diagnosed for each volunteer. Results Following screening, the Buffy coat (least recommended) technique did not detect the Leishmania parasite (0%), whereas the thin film examination (sensitivity: 2.3%, specificity: 99.6%, negative predictive value: 84.1%, positive predictive value: 50%) detected 2 (2.1%) while ELISA (the gold standard) detected 44 (16.2%) leishmaniasis cases among the participants. The results revealed statistically significant (p