Nano-partículas magnéticas para separación de espermatozoides X en semen equino. Resultados preliminares

DOMINGUEZ ESTEBAN; CLEMENTE, HENRY; CASTEX, HERNAN RAMÍREZ; BRAGULAT, ANA FLORES; UGAZ, CRISTIAN; GIOJALAS, LAURA; LOSINNO, LUIS

Pilarm Buenos Aires

Jornada; II JORNADA INTERNACIONAL DE BIOTECNOLOGIAS REPRODUCTIVAS EN EQUINOS; 2017

Pre-selection of sex before conception has been one of the objectivespursued by scientists and breeders for many years. Within dairy andmeat cattle industries there are productive reasons to calculate thefinancial benefit of preferring one sex. However, equines are subject toother variables i.e Polo horses where the industry prefers 100% mares(as in dairy cattle).The only replicable and efficient way up to now in separating thesperms that contain the X chromosome from those that contain the Ychromosome from a semen sample is through the use of cell sortingtechnique by high flow cytometer developed by Johnson in 1986(Samper et. al, 2012). However, it has only been used commercially andat big scale in dairy cattle industry. The number of spermatozoa thatcan be effectively selected per hour in the flow cytometer has been alimitation for its mass application, especially for species that requirea high number of sperms to inseminate a female, as it is the equinescenario (Panarace et. al, 2014). And also to consideration the level ofdamage that spermatozoa suffers during the process and the inabilityto get an appropriate frozen dose after sex sorting.The aim of this study was to test a new method of sex sortingspermatozoa that was also field friendly for using, none or less damagefor spermatozoa and faster than cytometer. The use of magnetic beadsin the nano scale could accomplish these features. The results showedthat sperm parameters where mainly not affected comparing to controlgroups (not exposed to nano particles) and fertility tests went well onour first small scale inseminating mares.