Cardiac Cells Embedded in Hydrogel Structured by a Dual Extruder Bioprinter

SANCHEZ DE LA NAVA ANA; CLIMENT ANDREU; NIEVA EDUARDO GABRIEL; SALVATIERRA NANCY

Córdoba

Congreso; XXI Congreso Argentino de Bioingeniería; 2017

Sociedad Argentina de Bioingeniería

The exponential development of the tissue engineering and their fields allow us to aim future therapies in chronical pathologies. The hydrogels versatility and the 3d printing rediscovery let us to get new studies and results transferable to get solutions where we could not think in the past. The aim of this study is to demonstrate the viable production of cardiac structures through the use of a 3D bio-printer. To carry on this objective specific bioprinting softwares was developed from an open source 3D printer. In addition, in order to simultaneously print two cell types, a duel extruder control was included. To get the cardiac structures a cardiac cell line HL1 was embedded in the alginate hydrogel and the cross linker was CaCl2. Optimal configuration of alginate, calcium dichloride and cell concentration was identified by means the development of viability assays. Specifically Alamar Blue ®, fluorescence and confocal microscopy were applied to test the viability and the spatial resolution of the printer. The more promising hydrogels configuration were those obtained with the lower lumen needle (16G), 2% alginate, 0.1 M gelling agent concentration and 2000000 cell/ml cell concentration. This technique had a survival rate from the 85% of the cells after the 24 hr. Thus, the fusion of the 3D printer and the hydrogels as alternative to conventional cell culture techniques is really promising. The use of the alginate with calcium as hydrogel works as non-cytotoxic scaffold to cardiac cells, and their application could be used for biomedical study.