IIBYT   23944
INSTITUTO DE INVESTIGACIONES BIOLOGICAS Y TECNOLOGICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Collagen obtaining for 3D printing to create biological substitutes for biomedical applications
Autor/es:
JURCZYSZYN SANTIAGO; ADRAGNA CELESTE; COMIN ROMINA; DIAZ NOCERA ADEN; SALVATIERRA NANCY ALICIA; CID MARIANA PAULA
Reunión:
Congreso; XXI Congreso Argentina de Bioingeniería X Jornada de Ingeniería Clínica; 2017
Resumen:
Biodegradable scaffolds with three-dimensionalporous structure are fundamental components for tissueengineering, on which cell cultures are grown to create biologicalsubstitutes that restore or repair tissue or organ damage.Collagen is the main protein of the human extracellular matrix,its structure allows the union, migration and in several cases, celldifferentiation; leading it to be one of the most studied materialfor the development of biomedical scaffolds. However, its use tofabricate reproducible and pore-structure-controlled 3Dstructures, which are designed to stimulate cell adhesion andsubsequent migration, and the easy diffusion of nutrients, hasbeen limited due to its low processability by the means of layerby-layertechnologies. In the present, we propose threedimensionalimpression for obtaining highly porous collagenscaffolds for biomedical use.A simple and economical method is developed for collagenpurification starting from porcine skin through treatments withacidic and salt solutions, which preserve the structure of thefibers unlike normally used enzymatic procedures whichdenature the protein. Characterization and quantificationthrough spectrophotometry and denaturing electrophoresisallowed to verify the effectiveness of this method for obtaininghighly pure type I collagen; and rheometric studies indicated theviscosity of the material was suitable for 3D printing applications.A collagen bioink was prepared, which presented appropriatedviscosity and stability for printing complex-structured 3Dscaffolds with an extrusion bioprinter. A physicalcharacterization of the printed scaffolds was performed through porosity and water swelling assays and cytocompatibilityaccording to the ISO Standard 10993, part 5-2009, indicatingthat they aren?t cytotoxic, as it is showN in this work. This leavesthe door open for subsequent studies oriented to cell cultureexperiments and in vivo analysis with the collagen printedscaffolds.