Caracterización biológica de composites de Titanio-Hidroxiapatita sinterizados a baja temperatura.

COMIN ROMINA; CID MARIANA PAULA; GRINSCHPUN LUCIANO; OLDANI CARLOS; TABORDA RICARDO; SALVATIERRA NANCY ALICIA

San Nicolás

Congreso; SABI 2015. XX Congreso de Bioingeniería - IX Jornada de Ingeniería Clínica.; 2015

Bone tissue loss caused by severe injury or disease is a critical problem in orthopedic clinics, and involves bone repairs and substitutes. At present, several devices and biomaterials in the treatment of these defectsare used. Increased interest in the use of titanium and hydroxyapatitecomposites for biomedical applications has been observed in recent years due to a good combination of the hydroxyapatite bioactivity and the favorable mechanical properties of metals. Powder metallurgyis a method that uses powder from titanium and hydroxyapatite to obtain composites having hydroxyapatite phases in metallic matrix. One of the steps of this methodincludes a sintering stage ranged 1000-1300ºC. However, in this system the presence of titanium accelerated dehydroxylation and the decomposition of hydroxyapatite to form tetracalcium phosphate and calcium oxideand these were detected at a temperature as low as 800°C. This imposes certain limitations on the thermal conditions for the manufacture of composites from a mixture of titanium and hydroxyapatite. In this work, we obtained a titanium-hydroxyapatite composite sintered at 800°C and evaluated its in vitrobiocompatibility. The MTT assay was carried out to assess cytotoxicity on Vero and NIH 3T3 cell lines in according to ISO 10993 Standard. In addition, the cell morphology and adhesion on composite surface wereanalyzed usingfluorescence and SEM microscopy. Our data show that the extracts from sintered composite at 800°C did not produce toxicity on Vero or NIH 3T3 cells neither affected the morphology and density of cells grown. Finally, the NIH 3T3 cells were adhered and proliferated on entire surface covering titanium-hydroxyapatite composite at day 4 of cell culture. These results provide in vitro evidence of good biocompatibility and bioactivity of this titanium-hydroxyapatitecomposite sintered at low temperature.