An in vitro model of SE induces neuronal death and changes in the levels of TrkB, pTrkB and p75ntr receptors in a mixed culture of neurons and astrocytes

VÍCTOR DANELON, ANDREA B. CRAGNOLINI, DANIEL H. MASCÓ

Córdoba

Congreso; Sociedad Argentina de Neurociencia , SAN, Huerta Grande, Córdoba; 2012

Previous studies from our laboratory showed that an in vitro model of status epilepticus (SE) (3 hours of deprivation of Mg+2) induces an increase in intracellular calcium, neuronal death and mobilizes TrkB and p75 receptors to the plasma membrane. In recent experiments using hippocampal neuron cultures, it has been observed that SE in vitro increases the levels of nitric oxide (ON) and induces a marked increase in levels of tubulin tyrosinated immediately after excitotoxic stimulation. In another series of experiments, it was characterized and evaluated the effect of the presence of astrocytes on neuronal death and on the levels of TrkB, pTrkB and p75ntr receptors after 3 hours of SE in vitro. It was observed, using immunocytochemical techniques, that there is a significant decrease in the number of nuclei, determined by DAPI and NeuN, 18 hours after the excitotoxic event. It was determined by Western Blot that SE in vitro induces a decrease in the levels of TrkB, pTrkB and p75ntr immediately after the excitotoxic event. It was observed that the levels of p75ntr receptor were recovered after 6 hours. Future xperiments will be necessary to determine the role of these events and of astrocytes in the neuronal death