Transcriptomics of early embryonic invasion at implantation sites in a murine model

MORENO-MOYA J.M.; FRANCHI, A.; MARTÍNEZ-ESCRIBANO S.; MARTÍNEZ-CONEJERO J.A.; BOCCA, S.; OEHNINGER, S.; HORCAJADAS, J.A.

San Diego

Congreso; Society for Gynecologic Investigation 2012 Annual Scientific Meeting; 2012

Society for Gynecologic Investigation

BACKGROUND: The embryonic invasion process requires two elements, one embryo that exerts the invasion and a maternal tissue that allows implantation and invasion. Disturbance in this dialogue is a potential source of serious pathologies such as pre-eclampsia and spontaneous abortion that can occur in early pregnancy. OBJECTIVE: The aim of this study is to break through the transcriptomic of the early embryonic invasive process and the maternal control exerted by the surrounding decidual tissue. DESIGN: Three pregnant female mice at 6.5 dpc were used to obtain tissue from invasive extra-embryonic tissue (ET) formed by the ectoplacental cone and two different decidual tissues, the surrounding decidua (SD) and the deciduas from the inter-implantation sites (ID). MATERIALS AND METHODS: Gene expression was analyzed using gene expression microarray technology and RT-PCR was performed for microarray validation. DAVID and IPA softwares were used to analyze the Gene Ontology (GO) terms most represented in the differentially expressed genes. RESULTS: Eight hundred seventeen genes were found to be differentially expressed between the ET versus SD and genes between ID versus SD. Both comparisons shared 123 genes. Expression of 8 of the most differentially expressed genes was confirmed by RT-PCR. GO analyses showed that developmental and proliferation processes were over-represented in the differentially expressed genes in ET vs SD and immune cell trafficking in ID vs SD. A very interesting network of genes involved in replication, recombination and cell repair, cell death and cell growth was found in the comparison ET vs SD. CONCLUSIONS: Our results provide closest information to humans about the transcriptome of the early embryonic invasion process at the implantation site and the control exerted by the surrounding decidual tissue. These results would be useful to find targets involved in different pathologies associated with implantation failure or early pregnancy loss. SUPPORT Ministerio de Ciencia e Innovación of the Spanish Goverment (SAF2008-04349).