IIBYT   23944
INSTITUTO DE INVESTIGACIONES BIOLOGICAS Y TECNOLOGICAS
Unidad Ejecutora - UE
artículos
Título:
Engineering Protein Nanoparticles Out from Components of the Human Microbiome
Autor/es:
LÓPEZ LAGUNA, HÈCTOR; VOLTÀ DURÁN, ERIC; UNZUETA, UGUTZ; VÁZQUEZ, ESTHER; SÁNCHEZ GARCÍA, LAURA; SÁNCHEZ, JULIETA M.; LOS, MARCIN; SERNA, NAROA; SÁNCHEZ CHARDI, ALEJANDRO; VILLAVERDE, ANTONIO
Revista:
SMALL
Editorial:
WILEY-V C H VERLAG GMBH
Referencias:
Año: 2020 vol. 16
ISSN:
1613-6810
Resumen:
Because of their low interactivity with human body components and their toleratedimmunogenicity, proteins derived from the human microbiome are appealing, fullybiocompatible building blocks for the biofabrication of inert protein materials to be usedin systemic application as drug carriers and in other clinical uses. Several phage andphage-like bacterial proteins with natural structural roles have been produced inEscherichia coli as polyhistidine-tagged recombinant proteins, pursuing theirassembling as discrete, nanoscale particulate materials. While all of them selfassembledin a variety of sizes, their stability at 37 ºC was found to be severelycompromised. However, by selectively adjusting temperature and Zn 2+concentration we favored the formation of robust nanomaterials, fully stable in complexmedia and under physiological conditions. On these basis, microbiome-derivedproteins show promises for the regulatable construction of scaffold protein materials,which can be tailored and strengthened by a simple physicochemical fine-tuning.