IIBYT   23944
INSTITUTO DE INVESTIGACIONES BIOLOGICAS Y TECNOLOGICAS
Unidad Ejecutora - UE
artículos
Título:
Sperm Sexing Mediated by Magnetic Nanoparticles in Donkeys, a Preliminary In Vitro Study
Autor/es:
DOMÍNGUEZ, ESTEBAN; BRAGULAT, ANA FLORES; GIOJALAS, LAURA; MORENO-IRUSTA, AYELEN; UGAZ, CRISTIAN; LOSINNO, LUIS; MORENO-IRUSTA, AYELEN; UGAZ, CRISTIAN; LOSINNO, LUIS; CASTEX, HERNAN RAMÍREZ; CLEMENTE, HENRY; CASTEX, HERNAN RAMÍREZ; CLEMENTE, HENRY; DOMÍNGUEZ, ESTEBAN; BRAGULAT, ANA FLORES; GIOJALAS, LAURA
Revista:
JOURNAL OF EQUINE VETERINARY SCIENCE
Editorial:
ELSEVIER SCIENCE INC
Referencias:
Año: 2018 vol. 65 p. 123 - 127
ISSN:
0737-0806
Resumen:
Preselection of sex before conception has been one of the objectives pursued by scientists and breeders for many years. The donkey milk industry has shown a greater commercial interest, since jennies? demand is increasing nowadays. Therefore, to get a female animal, the oocyte must be fertilized with previously sexed semen. The current technique used for sperm sexing separates spermatozoa containing the X or Y chromosome based on each cell DNA content. However, this technique exposes spermatozoa to high-pressure speed flow, DNA fluorescent stain, and UV light, factors that may affect sperm quality. The aim of this study was to test a new technique to isolate spermatozoa carrying the X chromosome by means of magnetic nanoparticles (MNPs). Results show that the MNP technique is highly effective in select X spermatozoa, without affecting several physiological sperm parameters. In conclusion, the MNP technique provides an X sperm population with similar sperm physiological state than the control sample. The new sexing technology presented here can be mainly applied to improve the donkey´s milk industry.