IIBYT   23944
INSTITUTO DE INVESTIGACIONES BIOLOGICAS Y TECNOLOGICAS
Unidad Ejecutora - UE
artículos
Título:
Assessment of Sperm Function Parameters and DNA Fragmentation in Ejaculated Alpaca Sperm (Lama Pacos) by Flow Cytometry.
Autor/es:
CHEUQUEMÁN C; MERINO O; GIOJALAS L; VON BAER A; SÁNCHEZ R; RISOPATRÓN J
Revista:
REPRODUCTION IN DOMESTIC ANIMALS (1990)
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Lugar: Londres; Año: 2013 vol. 48 p. 447 - 453
ISSN:
0936-6768
Resumen:
Flow cytometry has been shown to be an accurate and highly
reproducible tool for the analysis of sperm function. The main
objective of this study was to assess sperm function parameters
in ejaculated alpaca sperm by flow cytometry. Semen samples
were collected from six alpaca males and processed for flow
cytometric analysis of sperm viability and plasma membrane
integrity using SYBR-14⁄PI staining; acrosomal membrane
integrity using FITC-conjugated Pisum Sativum Agglutinin⁄PI
labelling; mitochondrial membrane potential (Dwm) by staining
with JC-1 and DNA Fragmentation Index (DFI) by
TUNEL. The results indicate that the mean value for sperm
viability was 57 ± 8 %. Spermatozoa with intact acrosome
membrane was 87.9 ± 5%, and viable sperm with intact
acrosomal membrane was 46.8 ± 9%, high mitochondrial
membrane potential (Dwm) was detected in 66.32 ± 9.51% of
spermatozoa and mean DFI value was 0.91 ± 0.9%. The DFI
was inversely correlated with high Dwm (p = 0.04; r = 0.41)
and with plasma membrane integrity (p = 0.01; r = 0.47). To
our knowledge, this is the first report of the assessment on the
same sample of several parameters of sperm function in
ejaculated alpaca sperm by flow cytometry.