A natural antioxidant attenuates the vascular calcification in a rat model of type 1 Diabetes mellitus

RIVOIRA M; RODRÍGUEZ V; MASSHEIMER V; RAUSCHEMBERGER M; TOLOSA DE TALAMONI N

Mar del Plata

Congreso; Reunión Anual SAIC-SAI-SAFIS; 2018

Vascular calcification (VC) is one important complication of type 1 Diabetes mellitus (D.M.). Several studies suggest that the antioxidant naringin (NAR) supplementation is beneficial for treatment of D.M, but its effect on the VC has not been investigated. The aim of this work was to know whether NAR could attenuate the VC in Wistar male rats with D.M. Three groups of animals were used: 1) controls, 2) diabetic rats (treated with 60 mg streptozotocin /kg b.w.: STZ), 3) diabetic rats treated with NAR (40 mg/kg b.w.). After 30 days of treatment, plasma was withdrawn and rats were sacrificed to obtain the aortas. Endothelial cells (EC) from aortas were cultured and NO?, indicator of vascular health, was measured by the Griess´s method. NO? production was significantly reduced in STZ rats, which was highly blocked by NAR (213,40 ± 33.3; 143,69±19.88*; 184,66±11.99; C; STZ; STZ + NAR 40; *p