INICSA   23916
INSTITUTO DE INVESTIGACIONES EN CIENCIAS DE LA SALUD
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
THE GPCR-Gai REGULATED THE FGF2-INDUCED LACTOTROPH AND SOMATOTROPH CELL PROLIFERATION
Autor/es:
PICECH F; TORRES A I; DE PAUL A L; SOSA L; PETITI JP
Lugar:
Mar del Plata
Reunión:
Congreso; LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; 2018
Resumen:
In a previous study, we demonstrated that the co-incubation of basic fibroblast growth factor (FGF2) with the analog of somatostatin, octreotide (OCT) induced G1-phase arrest in pituitary cells, suggesting that the somatostatin receptors, inhibitory G protein-coupled receptors (GPCR-Gαi), may regulate the FGF2 effects. The aim was to evaluate whether the FGF2 proliferative activity is regulated by GPCR-Gαi specifically in lactotroph and somatotroph cell population. Anterior pituitary cell cultures from female rats were treated with FGF2 (10 ng/mL) or OCT (100mM) alone or co-incubated with or without pertussis toxin (PTX, 500nM), an inhibitor of GPCR-Gαi. The lactotroph and somatotroph cell proliferation was analyzed by double-immunocytochemistry of BrdU and PRL or GH at 24 and 48h. The p-ERK1/2, c-Jun and cell cycle regulation proteins: cyclin D1, CDK4, p21 and p27 were determined by western blot. Statistics: ANOVA-Bonferrony. The percentage of BrdU/lactotroph positive cells was 7.2% and BrdU/somatotroph positive cells was 3.4%. The lactotroph and somatotroph cell proliferation was increased by FGF2 whereas OCT decreased the cell mitosis respect to control group. The FGF2/OCT co-incubation significantly decreased the proliferation in both cells types after 24 and 48 h respect to FGF2 group, effect that was reverted by pre-incubation with PTX. The diminution in the cell proliferation was associated with an increase in the cell cycle inhibitors p27 and p21 expression, and a decrease of cycle D1 while the CDK4 did not show any significant variation. In addition, a remarkable decrease of pERK1/2 and c-Jun expression was observed after combined treatments.