INICSA   23916
INSTITUTO DE INVESTIGACIONES EN CIENCIAS DE LA SALUD
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Basal and Hyperosmolality-induced AVP Expression is Afected by Erly Maternal Separation
Autor/es:
VERÓNICA TRUJILLO; LAURA VIVAS; FRANCO R. MIR, ; , MARTA SUÁREZ
Lugar:
BUENOS AIRES
Reunión:
Congreso; Reunión Conjunta de Sociedades de Biociencias-; 2018
Institución organizadora:
SAIC
Resumen:
BASAL AND HYPEROSMOLALITY-INDUCED AVP EXPRESSION IS AFFECT BY EARLY MATERNAL SEPARATION Franco Rafael Mir (1, 2), Verónica Trujillo (3), Laura Vivas (4, 5), Marta Suárez (3) (1) Cátedra de Fisiología Animal, FCEFyN, Universidad Nacional de Córdoba. (2) Cátedra de Fisiología Animal, DCEFyN, Universidad Nacional de La Rioja. (3) Cátedra de Fisiología Animal, FCEFyN, Universidad Nacional de Córdoba, Instituto de Investigaciones en Ciencias de la Salud (INICSA). (4) Laboratorio de Balance Hidrosalino e Hipertensión. (5) Instituto de Investigación Médica Mercedes y Martín Ferreyra (INIMEC-CONICET-Universidad Nacional de Córdoba). We assess the implication of an adverse early environment such as the maternal separation (MS) on the regulatory response to osmotic stress during adulthood. Since the interplay between an early psychological stress and late osmotic stress both associated with the activity of vasopressinergic circuits within the paraventricular (PVN) and the supraoptic (SON) nuclei would result in a differential osmoregulatory response in terms of vasopressin (AVP) mRNA levels during adulthood. The aim of this work was to evaluate whether early MS may induce a differential programming in the adult offspring vasopressinergic system in particular the hyperosmolality-induced AVP expression. Male Wistar rats were subjected to daily maternal separation for 4.5 hours during the first three weeks of life. At postnatal day 75, rats were intravenously infused with isotonic or hypertonic saline solution during 20 minutes. After 10 minutes, animals were decapitated. Thereafter, the PVN and SON were identified and collected by micropunch technique. Then, we determined the relative AVP expression by qPCR. In the PVN, as we expected, non-separated animals responded to hypertonic stimulation with a threefold increase in AVP levels (p