Sexual dimorphism in the response of human neutrophils to LDL

MALDONADO C; SCALERANDI MV; SOEHNLEIN O; ORTEGA-GOMEZ A; QUINTAR AA

Mar del Plata

Congreso; LXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; 2018

Sociedad Argentina de Investigación Clínica (SAIC)

Hyperlipidemia milieu, characterized mainly by elevated low-density lipoprotein (LDL) cholesterol levels, activates endothelial and circulating cells which promotes leukocyte infiltration to large arteries and atherosclerosis development. In addition to the well-studied role of monocytes/macrophages, emergent evidence indicates a pathogenic role for neutrophils during various stages of atherosclerosis. Although there is a atherosclerosis-associated male:female mortality ratio 2:1, it remains to be elucidated if sexual differences occur in neutrophil response to atherogenic conditions. Therefore, our objective was to determine the sexual differences in the LDL-induced neutrophil activation. Human neutrophils were isolated from peripheral blood of healthy young men and women (18?40 years old) using Polymorphprep?, resuspended at 1-5x105 cells/ml and incubated at 37°C, 5% CO2 with LDL (100 ug/ml) or vehicle for 2 to 8 hs to evaluate the surface expresion of CD11b, CD16, CD11a, CD29, CXCR2, CXCR4, and CD62L by FACS, functional static adhesion assay to endothelial adhesion molecules (P-selectin, ICAM1 and VCAM1), and phagocytosis of FITC-labeled latex beads by FACS. Statistical analysis: Paired t test, p