INICSA   23916
INSTITUTO DE INVESTIGACIONES EN CIENCIAS DE LA SALUD
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
STUDY OF SAFETY OF EXTRACTS OBTAINED FROM Arachis hypogaea L (PEANUT)
Autor/es:
SUAREZ PERRONE AGOSTINA; SABINI MARÍA CAROLA; MENIS CANDELA FLORENCIA; GIORDANO WALTER; ESCOBAR FRANCO MATÍAS
Lugar:
Mendoza
Reunión:
Congreso; XXXVI REUNIÓN CIENTIFICA ANUAL DE LA SOCIEDAD DE BIOLOGÍA DE CUYO; 2018
Institución organizadora:
Sociedad de Biología de Cuyo
Resumen:
The peanut plant (Arachis hypogaea L) is a legume that belongs to Fabaceae family and Arachis genus. It is native to South America and is grown around the world in tropical, subtropical and temperate regions. The plant contains several active components that include flavonoids, phenolic acids, phytosterols. Scientific reports have indicated that these components can cause various biological effects, including cardio-protective, anti-inflammatory, antioxidant, anti-cancer, antimicrobial, anti-diabetic, anti-obesity and sedatives. There are no reports on toxicity. The aim of this work was to evaluate the ethanolic extracts of Arachis hypogaea L in its cytotoxic and genotoxic capacity in vivo. For this, ethanol extracts of seeds (EES) and tegument (EET) of peanuts were obtained by a simple alcohol extraction method. Cytotoxicity studies: Vero cell monolayers were treated with increasing concentrations (0-1400 μg/ml) of EES and EET. Cytotoxicity was determined at 48 h of incubation by: Neutral Red (NR) uptake and MTT reduction. For the genotoxicity studies, the Micronucleus Test was carried out in mouse bone marrow. Healthy Balb/c mice of 2 months age (25 g) were used. They received, by intraperitoneal injection, 0.2 ml of EET at concentrations of 25, 50, 100 and 250 mg/Kg of body weight (b.w.) and of EES at the concentrations of 500, 1000 and 2000 mg/Kg b.w. prepared in physiological solution and DMSO (Dimethylsulfoxide). Negative (NC), positive (PC) and vehicle (VC) controls were included. The animals were sacrificed by cervical dislocation at 24 h post-injection. The marrow samples from the femoral bones were spread on slides, stained with May-Grünwald-Giemsa solution and observed under a microscope. The genotoxicity index was determined: number of MNPCE (micronucleated polychromatic erythrocytes) in a total of 1000 PCE (polychromatic erythrocytes) per spread and the toxicity index (TI), considering the PCE/NCE ratio (normochromatic erythrocytes) every 1000 PCE. The cytotoxicity results indicated CC50 values of 600 μg/ml (MTT) and >1600 μg/ml (NR) for EET and >1400 μg/ml (MTT) and 1600 μg/ml (NR) for EES. EES was less toxic than EET by the MTT technique. The genotoxicity indexes were 6, 8.5, 9, 10.7 and the TI: 1.68, 1.47, 1.43, 0.95 for the concentrations 25, 50, 100 and 250 mg/Kg of EET, respectively. The EES presented as genotoxicity indexes: 7.5 (500 mg/Kg), 7.4 (1000 mg/Kg) and 9.6 (2000 mg/Kg) and as TI: 1.08 (500 mg/Kg), 1.31 (1000 mg/Kg), 1.95 (2000 mg/Kg). The genotoxicity indexes were for NC: 8.5, PC: 27 and VC: 8.25. TI: 1.32 (NC), 1.5 (PC), 1.12 (VC). None of the extracts were found to be genotoxic (p>0.05). Only the highest concentrations of EET and EES showed some toxicity, with respect to the negative control (p