INICSA   23916
INSTITUTO DE INVESTIGACIONES EN CIENCIAS DE LA SALUD
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
In vitro study of Amphotericin-B and Clomipramine combination upon Leishmania braziliensis
Autor/es:
STRAUSS M; KONIGHEIM B; BAZAN PC; PAGLINI PA; AGUILAR J; LO PRESTI MS; VELÁZQUEZ D; LOZANO E; AMELIA E; BAEZ A; RIVAROLA HW
Lugar:
Bs As
Reunión:
Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017
Institución organizadora:
Sociedad Argentina de Protozoología (y otras)
Resumen:
Current drugs for the treatment of Leishmaniasis show disadvantages due to toxicity and high cost. Among these, Amphotericin-B (AMPHO), which causes the death of the parasites due to alterations in theircell membranes, also has adverse effects upon the host. Consequently, the search for new drugs,focused on molecular targets that are present in the parasite but absent in the host,continues. Clomipramine (CLO) is a potent inhibitor of trypanothione reductase, an enzyme exclusive to theKinetoplastida order.The aim of the present work was to analyze the in vitro combined effect of AMPHOand CLO upon L. braziliensis promastigotes. We performed a cytotoxicity assay on Vero cellsby incubating 2.5x105 cells/mL with AMPHO= 3.25?50 μg/mL and CLO = 7.8?250 μg/mL for 4 days, and a combined effect assay in which 3x106 promastigotes/mL were incubated with AMPHO= 0.002?0.032 μg/mL and CLO = 0.3?2 μg/mL.We determined the IC50 values,calculated the Combination Index (CI: CI>1 antagonist, CI=1 additive, CI