OMEGA-3 FATTY ACID MEDIATE IMMUNEREGULATION ANTICANCER MECHANISMS BY IL-6, 5-HETE AND ROS GENERATION IN PANCREATIC HUMAN CELLS

MARÍA ISABEL GARAY, MABEL BRUNOTTO, BELÉN RABAGLINO, NELSO N BAROTTO , PATRICIA L QUIROGA, GASTÓN REPOSSI , MARTÍN E FERNÁNDEZ-ZAPICO, MARÍA EUGENIA PASQUALINI

Congreso; SAIB; 2017

Pancreatic carcinogenesis is characterized by the activationof inflammatory signaling pathways. Numerous studies havedemonstrated the role of essential fatty acids (EFAs) in pancreaticcancer initiation and progression, but the underlying molecularmechanisms have not been completely elucidated. Here, we studiedthe effects of two omega-3 EFAs, the eicosapentaenoic acid (EPA)and docosahexaenoic acid (DHA) and the omega-6 fatty acid, thearachidonic acid (AA) on a human pancreatic cell line (PANC-1).We determined the release of interleukin-6 (IL-6) as inflammatorymarker in supernatants from PANC-1 cultures using ELISA, as wellas their gene expression by qRT-PCR. Cell viability (Rezasurin) andreactive oxygen species (ROS) production (DCFH-DA assay kit)by fluorimetry. Eicosanoids generation and lipid cell profile by Highpressure liquid and Gas chromatography (HPLC and GC).We observeda significant reduction in cell viability in DHA and EPA comparedto AA and control (ethanol) treatments (P