Non-classical testosterone signaling implications in prostate smooth muscle cell proliferation and muscle cell phenotype.

LEIMGRUBER, C; NICOLA, JP; CUELLO RUBIO, M; PEINETTI, N; QUINTAR, AA; SCALERANDI, MV; MALDONADO, CA

Mar del Plata

Congreso; LXI Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC).; 2016

Sociedad Argentina de Investigación Clínica (SAIC).

Testosterone (T) effects are mediated by a classical pathway that takes place through the binding of T to the classical androgen receptor (AR) and migration to the nucleus, and by non-classical signaling pathways which are mediated by cell surface receptors capable of activating signaling cascades. The differentiation and proliferation of pSMC as well as the stroma/epithelium interaction are essential in the development of prostatic pathologies. Ourobjectives were to determine the presence of plasma membrane AR receptors in pSMC and their participation in cell differentiation, proliferation and growth factors expression. pSMC were obtained from Wistar rat prostate and stimulated in vitro with T 10-7M or the plasma membrane impermeable T-bovine serum albuminconjugate (T-BSA) 10-7M. Smooth muscle markers and the expression of FGF7 and TGFβ were evaluated by qPCR, and cell proliferation was assessed by immunocytochemistry of Ki67, and confirmed by cell counter; statistical analysis was performed by ANOVA-Tukey. The AR plasma membrane localization was demonstrated by immunofluorescence and confocal microscopy, and by co-localization with the membrane marker concanavalin-A. This assay was verified by flow cytometry, which showed a population of membrane AR positive cells of 18.87 ± 2.43%. After T-BSA stimuli, an increased expression of smooth markers calponin and α-actin was observed (p