INICSA   23916
INSTITUTO DE INVESTIGACIONES EN CIENCIAS DE LA SALUD
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
COCAINE-INDUCED MICROGLIA ACTIVATION IN NUCLEUS ACCUMBENS AND CAUDATE-PUTAMEN IS REVERSED BY NALOXONE
Autor/es:
BARTOS M.; MONGI BRAGATO B.; CANCELA LM.; AVALOS M. P.; IRIBARREN P.
Lugar:
Cracovia
Reunión:
Conferencia; 22nd Annual Scientific Conference - Society on NeuroImmune Pharmacology.; 2016
Institución organizadora:
Society on NeuroImmune Pharmacology.
Resumen:
Previous findings from our lab show a long-lasting psychostimulant-inducedsensitization phenomenon at the immune level in a manner parallel to that occurring in the limbic and immune enkephalinergic systems (Assís et al., 2006, 2009, 2011). We also demonstrated that the enkephalinergic system is essential for behavioral and molecular sensitization to cocaine within the nucleus accumbens (NAc) and caudate putamen (CPu) (Mongi-Bragato et al., 2014). However, there is no description so far of how microglia is involved in psychomotor sensitization and how the enkephalinergic system participates in this. We treated male C57B/6J mice daily with naloxone (1 mg/kg i.p.) or vehicle previousto cocaine (15 mg/kg i.p.) and vehicle for 9 days, followed by a cocaine challenge (7.5 mg/kg i.p.) on day 21 of the treatment. The immunohistochemistry was performed in the areas of interest using CD11b and met-ENK antibodies. Cytokines were measured by qRTPCR. In the control treatment, microglia cells had small soma and ramified processes. Repeated administration of cocaine induced morphological changes of microglial cells indicative of cell activation and also increased production of pro-inflammatory cytokines such as TNF-α, which was reversed by naloxone pretreatment. These preliminary results could be key to a better understanding of the role of the enkephalinergic system in the immunological signaling system in drug addiction and may provide a new therapeutic target.