INICSA   23916
INSTITUTO DE INVESTIGACIONES EN CIENCIAS DE LA SALUD
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Tissue-specific bioconcentration of cypermethrin, chlorpyrifos and its mixture in a native fish (Jenynsia multidentata).
Autor/es:
BONANSEA R.I; MARINO D.J.; WUNDERLIN D.A.; AMÉ, M. V.
Lugar:
Buenos Aires
Reunión:
Congreso; 11° Meeting SETAC-LatinAmerica; 2015
Institución organizadora:
SETAC
Resumen:
Chlorpyrifos (CPF) and Cypermethrin (CYP) tissue distribution, bioconcentration and biotransformation of these insecticides were investigated in J. multidentata as a model. For this aim, adult female fishes were exposed 96 h to 0.04 μgL-1 of CYP and 0.4 μgL-1 of CPF singly and in technical mixture. Additionally, the same mixture of a commercial product was tested. CPF and CYP concentration in liver, gill, brain, intestine, muscle and gonads were analyzed by GC-ECD and confirmed by HPLC-ESI-QTOF;MS. The activity of Gluthatione S-transferase was measured by spectrophotometry, while the expression of cytochrome P4501A (CYP1A) and P-glycoprotein multidrug transporter protein (P-gp) was measured by Reverse Transcription q PCR. Fish exposed to CYP accumulate this compound only in muscle with a bioconcentration factor (BCF) of 225. Possible reasons may include lesser biotransformation capacity of muscle and the activation of CYP1A expression in the liver when compared to the control (p< 0.05). On the contrary, fish exposed to CPF showed the accumulation of the insecticide at entry points, intestine (BCF= 168) and gills (BCF=105), and in the liver (BCF= 145), located in a strategic position within the organisms. Although fish exposed to CPF have higher levels of GST in gills and P-gp in liver when compared to control group (p< 0.05), the accumulation occurred. Fish exposed to the technical mixture showed CYP accumulation with the following distribution pattern: liver (BCF= 3900) > intestine (BCF= 1300) > gills (BCF= 200), with higher BCF for this treatment than BCF calculated in fish exposed to this insecticide singly. Higher expression levels of CYP1A in liver than control group was also observed (p< 0.05). Fish exposed to the commercial mixture bioconcentrated both insecticides, showing the following distribution pattern of CYP: intestine (BCF= 2900) > gills (BCF= 1300) and CPF was: liver (BCF= 808) > intestine (BCF= 125)> gills (BCF= 28)>muscle (BCF= 20). Moreover, in the organs where accumulation occurred, the biotransformation system was inhibited. These results demonstrated that highest bioaccumulation of CYP and CPF in J. multidentata occurs after commercial formulation exposure possibly associated to biotransformation system of fish overcome. Both insecticides accumulate in liver, gill, intestine and muscle fish tissues and that bioconcentration was different among diverse exposure conditions.