INICSA   23916
INSTITUTO DE INVESTIGACIONES EN CIENCIAS DE LA SALUD
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Linking cypermethrin and chlorpyrifos residues with toxicological effects on native fish Jenynsia multidentata: a laboratory study.
Autor/es:
BONANSEA, R.; WUNDERLIN D.A.; AMÉ, M. V.
Lugar:
Buenos Aires
Reunión:
Congreso; 11° Meeting SETAC-LatinAmerica; 2015
Institución organizadora:
SETAC
Resumen:
The extensive use of the insecticides Cypermethrin (CYP) and Chlorpyrifos (CPF) in Argentina for agriculture and domestic purposes resulted in the presence of these pesticides in local rivers and the exposure of non-target organisms. Moreover, CYP and CPF residues were detected in different organs of the native fish Jenynsia multidentata exposed to these pesticides singly as well as in technical and commercial mixtures under laboratory conditions. The aim of the present study was to evaluate the toxic effects of CYP and CPF using biochemical and behavioral biomarkers when the J. multidentata was exposed to the same chemical stress. Moreover, we explore the linkage between the responses of biomarkers and the pesticides residues measured in fish tissues. Thus, adult female fishes were exposed over 96 h to 0.04μg/L CYP; 0.4μg/L CPF; 0.04μg/L CYP + 0.4μg/L CPF in a technical mixture; and 0.04μg/L CYP + 0.4μg/L CPF in a mixture of commercial products. Forty five variables were measured in different fish organs (biotransformation, antioxidant and, cholinesterase enzymes activities; P-glycoprotein and aromatase expression; oxidative damage in lipids and proteins) as well as several swimming behavior parameters. Then, exploratory multivariate analysis identified differences among treatments according to the biomarkers response. Linear discriminant analysis was further applied to identify 1 or 2 biomarkers per organ that better explain the variability among treatments. Those selected biomarkers responses were included in an Integrated Biomarker Response index (IBR) which allowed to arrange the treatments according to an increase in the effect on J. multidentata: Control < CYP 0.04μg/L < CPF 0.4μg/L < 0.04μg/L CYP + 0.4μg/L CPF in a technical mixture < 0.04μg/L CYP + 0.4μg/L CPF in a mixture of commercial products. Finally, a Spearman correlation analysis was performed between the IBR and the CYP and CPF concentrations estimated in total fish tissue. A significant correlation (r= 0.999, p< 0.05) was obtained between IBR and total pesticide concentration. This result indicates that the higher accumulation of the two insecticides, the greater the effect of toxicity found in J. multidentata. Even when positive correlations were found between IBR and residues of CYP or CPF, they were not statistically significant (CYP: r= 0.82, p= 0.09; CPF: r= 0.67, p= 0.2), thus we cannot attribute the effects to the bioaccumulation of only one pesticide.