Chronic consumption of high fat diet in mice: effects on body weight, reproductive physiology and impact on offspring

FERNÁNDEZ F; GASPAROTTO A; MAZZONI F; LUQUE EM; MARTINI AC; CARLINI VP; VINCENTI LM

Córdoba

Jornada; XVI Jornada de Investigación Científica de la Facultad de Ciencias Médicas; 2015

Secretaría de Ciencia y Tecnología, UNC

Excess of body weight presents associated diseases that can be transmitted to offspring by maternal or paternal route. Objective: to investigate effects of chronic consumption of high fat diet on body weight and reproductive physiology of adult mice and their descendants. Albino Swiss males and females mice were fed (10 weeks) with control (C-3.9% fat, 18% protein, 42% carbohydrates) or high fat diet (HF-24% fat, 24% protein, 41% carbohydrates) and then mated with untreated animals. It was evaluated: body weight (BW) during treatment in adults and in their offspring from birth to adulthood; in parents and male offspring: sperm functional activity (concentration, motility, bending forms, vitality, hypoosmotic swelling test, acrosome reaction); in all descendants: beginning of puberty (vaginal opening / testicular descent). Repeated measures ANOVA was used, considering statistically significant values of p