Expression and localization of enzymes Indoleamina 2,3-dioxygenase and Aryl hydrocarbon Receptor in Chagasic Placentas

MOREIRA ESPINOZA, M.J.; TRIQUELL, M.F.; MORAN, J.; PIEGARI, M.; MEZZANO, L.; DÍAZ LUJAN, C.; FRETES, R.E.

Bilbao

Congreso; VI INTERNATIONAL CONGRESS OF HISTOLOGY AND TISSUE ENGINEERING; 2015

SOCIEDAD ESPAÑOLA DE HISTOLOGIA E INGENIERIA TISULAR

Indoleamine 2,3-dioxygenase (IDO) is an intracellular enzyme, that is constitutively expressed in placenta. It is implicated in depleting Tryptophan (Trp). The product, kynurenines are substrate for Aryl Hydrocarbon Receptor (AhR) enzyme, which is also expressed in placenta. Both IDO and AhR are crucial for modulating immune response in pregnancy. This system is involved in the resistance to Trypanosoma cruzi infection, the causal parasite of the Chagas disease, in experimental conditions. Therefore, this system might be participating in the congenital transmission of the Chagas disease. Objective: to analyze the expression and localization of IDO and AhR in placentas from chagasic pregnant women (CPW). Methods: Paraffin-embedded placental tissues from healthy pregnancies (control) (PN) (n=5) and CPW (n=14), who transmitted (CHWT) (n=5) or not (CHNT) (n=9) the infection to their new born were used. Slices of placental tissue were stained with anti-IDO and anti-AhR by Immunohistochemistry. Primary antibodies were replaced for control of the technique. Immunodetection was analyzed at 40X and semi-quantified employing ImageJ software. Statistical analysis was performed using GraphPad Prism by Student´s t Test and ANOVA, with Bonferroni post-hoc differences with p-value of