Renal Oxidation of ESS diabetic rats is minimized by EPA

NESTOR HORACIO GARCIA; OSIEKI, NATALIA; DIAZ, GUSTAVO TOMAS; PECCHIO RUIZ, ADRIANA; ALDO R EYNARD; REPOSSI, GASTON

Amsterdam

Simposio; 7th International Symposium on Atherosclerosis; 2015

International Atherosclerosis Society

Salt sensitivity (SS) is associated with increased cardiovascular risk in diabetic patients due to increased renal oxydation and decreased urinary sodium excretion. Western diet has limited content of w3 polyunsaturated fatty acids, with antioxidant capacity, such as ecoisapentaneoico acid (EPA). Therefore we hypothesized that nutritional supplementation with EPA, prevents SS in DM rats by decreasing renal oxidative stress. Methods: Wistar rats were used as healthy controls. Type II diabetic rat group (eSS), 3 months old, were divided in 3 groups, diabetic control (eSS), eSS treated with arachidonic acid (pro-oxidant)(2.5mg/ip, monthly) (eSS+AA) and eSS treated with EPA (2.5mg/ip month) (eSS+EPA). Animals were treated during 1 year, then placed in metabolic cages and subsequently underwent 2 subsequent experimental periods of 7 days eachwith normal sodium diet (0.4% NaCl)(NNaD) and high salt diet (4% NaCl)(HNaD). At the end of each period, weight, systolic blood pressure (SBP), HbA1c, triglycerides (Trig), cholesterol (Chol), creatinine (Cre), kidney γ-glutamyl transpeptidase activity (γGTP), urinary protein excretion (UprotV) were assayed. Results eSS rats had elevated HbA1c, Tri, Chol and reduced body weight vs. Wistar control group. Renal function was normal all along the experimental period. The eSS+AA group also showed elevation of HbA1c, Trig, with no change in Cre and Chol. During NNaD SBP was 119±3mmHg and after HNaD 125±1mmHg (p