INICSA   23916
INSTITUTO DE INVESTIGACIONES EN CIENCIAS DE LA SALUD
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
High quantities of trypomastigotes of Trypanosoma cruzi produces placental structural alterations associated to oxidative stress in vitro.
Autor/es:
TRIQUELL, MARÍA FERNANDA; MOREIRA ESPINOZA, MARÍA JOSÉ; DÍAZ-LUJÁN, CINTIA; PIEGARI, MARIANA; MEZZANO, LUCIANA; MAZZUDULLI, GINA; FRETES, RICARDO E
Lugar:
Mar del Plata
Reunión:
Congreso; X Congreso de Protozoología y Enfermedades Parasitarias; 2014
Institución organizadora:
Sociedad Argentina de Protozoología
Resumen:
Placenta participates actively in the control of congenital Chagas infection, which would explain, the low transmission rate. Production of pro-inflammatory cytokines such as TNFα, promotes nitric oxide (NO) production by the placental endothelial Nitric Oxide Synthase (eNOS) that could play an important role of this control. NO reacts with oxygen species and produce peroxynitrites which are both trypanosidal and potentially deleterious for placental tissue. Objective: To analyze structural alterations of the syncytiotrophoblast (STB), induced by two populations of T. cruzi through increased TNFα, NOS and oxidative stress. Placental villi explants co-cultured for 24 hs with 1x105 and 1x106 trypomastigotes of Tulahuen and Lucky strains (isolated from a congenital case); controls without parasites, and with addition of eNOS inhibitor L-NAME (1mM, 0,1mM, 0,01 mM). Histological quantification of the detachment area of STB. In culture media: quantification of NO (Griess), TNFα (ELISA).Tissue homogenates: NOSe and Nitrotyrosine expression by ELISA; and Gamaglutamiltranspeptidase (GGT) activity to measure oxidative stress. Placental explants in presence of both strains of T. cruzi showed a significant rise of TNFα, tyrosin nitrosilation, eNOS expression and GGT activity. The structural alterations (STB detachment) were significantly higher in presence of T. cruzi in a concentration dependent manner. L-NAME prevents a rise of detachment with T.cruzi. The presence of high quantity of T. cruzi in vitro produced detachment of the STB, associated with increased production of TNFα, eNOS and oxidative stress, which could facilitate the chorionic villi infection. These results could explain the association of some clinical aspects of the congenital Chagas disease, with structural alterations described in placentas from chagasic women. These results would constitute a new possible route of placental infection.