Effects of dietary chia oil on growth parameters in a syngenic murine model of breast adenocarcinoma

VARA MESLER M; PASQUALINI M E; COMBA A; SILVA RA; EYNARD AR; VALENTICH MA

Santa Fe

Jornada; VI Jornadas De Bioquimica Y Biologia Molecular De Lipidos Y Lipoproteinas; 2014

Facultad de Bioquímica y Ciencias Biológicas - Universidad Nacional del Litoral

Epidemiological data link ω?3 polyunsaturated fatty acid (PUFA) consumption to lower incidence of breast cancer (BC). Chia oil (ChO) is rich in α? linolenic acid (ALA ω?3), while corn oil (CO) is rich in linoleic acid (LA ω?6). These PUFAs give rise to eicosanoid with opposite effects. ω?3 PUFAs generate anti? inflammatory eicosanoids and reactive oxygen species (ROS), which could affect NF? κB, a transcription factors with a dual role in tumorigenesis. We aimed to study cellular processes activated by ChO diet on BC. Methods: BALB/c mice were fed with Chia Oil (ω?3) or Corn Oil (ω?6). Animals were inoculated with a mouse cell line (LM3) and tumor parameters were recorded. PUFAs incorporation into cell membranes was analyzed by GC, whereas eicosanoid production by HPLC. Western blot was performed to detect p?IκBα/ IκBα, as an index of NF?κB activation. ROS release was evaluated by flow cytometry in cultured LM3 cells after treatment with LA or ALA. Results: Tumor incidence was higher in CO compared with ChO?fed mice. Tumor weight, volume and metastasis number were lower, whereas tumor latency time was higher in ChO?fed mice (p