Effect of litocholic acid and sodium deoxycholate on intestinal calcium absorption

MARCHIONATTI AM; PEREZ A; RIVOIRA M.A; RODRÍGUEZ V; TOLOSA DE TALAMONI N

Bs As

Congreso; VIII Society for Free Radical Biology and Medicine South American Group.; 2013

Bile acids and their salts are the major components of bile. Litocholic acid (LCA) and sodium deoxycholate (NaDOC) are secondary bile acids. In this laboratory, we have demonstrated that the NaDOC, by an oxidative mechanism, inhibits the intestinal calcium absorption affecting mitochondrial membrane and cell survival (Rivoira y col. Comp Biochem Physiol 2012). The objective of this work was to investigate the effect of a single dose of LCA on the intestinal trancellular calcium pathway. Four-week old chicks were used: 1) controls, 2) treated with NaDOC, 3) treated with LCA and 4) treated with LCA + NaDOC. The intestinal Ca2+ absorption, protein carbonyl content, changes in mitochondria membrane permeability, Ca2+ pump, Na+/Ca+2 exchanger and calbindin D28K protein and gene expressions were analyzed. LCA alone did not alter the intestinal Ca2+ absorption. The combined treatment restored the inhibitory effect caused by NaDOC on the intestinal Ca2+ absorption and returned the values of the protein and gene expression to control. LCA avoided the increment in the protein carbonyl content and altered the mitochondrial membrane permeability produced by NaDOC. In conclusion, LCA prevented the effect produced by NaDOC on the trancellular Ca2+ pathway at least in part by blocking of the oxidative stress.