Daily variations of GPDH transcripts profile in Triatoma infestans flight muscles

MARÍA M. STROPPA; MARIANA S. LAGUNAS; CARLOTA S. CARRIAZO; BEATRIZ A. GARCÍA; NELIA VERMUTH; NELIA M. GEREZ DE BURGOS

Tunuyán (Mendoza)

Simposio; XII Latin American Symposium on Chronobiology; 2013

Latin American Group of Chronobiology

Triatoma infestans (T. infestans), main vector of Chagas disease, is a blood-sucking insect. Adults flight dispersal is the most important mechanism for reinfestation of houses after insecticide spraying. Flight muscles have two glycerol-3-phosphate dehydrogenase (GPDH) isoforms: GPDH-1 is involved in flight metabolism and GPDH-2 provides lipid precursors. The isoforms exhibit temporal expression patterns, sex-differentiated, and changes in relation to temperature and the amount of intake. In this work, we explored the transcripts profile of GPDH isoforms at 12, 16, 20, 24, 4 and 8 h, in adult flight muscles under light:dark cycle(LD), constant light(LL) and constant dark(DD). Semiquantitative RT-PCR investigations revealed that transcripts profile has daily variations according to the sex. In LD group, females had GPDH-1 transcript daily constant between 12 to 4h and absent at 8h; In males, GPDH-1 transcript was only expressed at 24h; The GPDH-2 transcript was absent in males and was expressed in females at 12 and 20h. In LL group, the GPDH-1 transcript profile was the same for females and males, expressed at 12, 20, 24 and 8h; the GPDH-2 transcript was only obserbed in females at 8h. In DD group, females had the same profile for both transcripts, expressed at 20 y 8h. In males the GPDH-2 transcript was absent and GPDH-1 was only expressed at 16h. These results show the daily variations in the GPDH transcripts profile that could correspond to a circadian rhythm.