INICSA   23916
INSTITUTO DE INVESTIGACIONES EN CIENCIAS DE LA SALUD
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Melatonin is a good antioxidant to avoid inhibition of intestinal calcium absorption caused by menadione
Autor/es:
ARECO V, CARPENTIERI A, MARCHIONATTI A, PEREZ A, CENTENO V, TOLOSA DE TALAMONI N,
Lugar:
Ciudad Autónoma de Buenos Aires
Reunión:
Congreso; VIII Meeting of the society for free Radical Biology and Medicine; 2013
Resumen:
The intestinal calcium (Ca2+) absorption is inhibited by menadione (MEN) through oxidative stress and apoptosis. The aim of this study was to elucidate whether the antioxidant and antiapoptotic properties of melatonin (MEL) could protect the gut against the oxidant MEN. For this purpose, 4 week old chicks were used. Oxidative stress was assessed by determination of glutathione (GSH) and protein carbonyl contents as well as antioxidant enzyme activities. Apoptosis was assayed by the TUNEL technique, protein expression and activity of caspase-3. The data show that MEL restores the intestinal Ca2+ absorption altered by MEN. In addition, MEL reversed the effects caused by MEN such as decrease in GSH levels, increase in the carbonyl content, alteration in mitochondrial membrane permeability and enhancement of superoxide dismutase and catalase activities. Apoptosis triggered by MEN in the intestinal cells was arrested by MEL, by normalization of the mitochondrial membrane permeability, caspase-3 activity and DNA fragmentation. In conclusion, MEL reverses the inhibition of intestinal Ca 2+ absorption produced by MEN counteracting oxidative stress and apoptosis. MEL could be a potential drug of choice for the reversal of impaired intestinal Ca 2+ absorption in certain gut disorders that occur with oxidative stress and apoptosis.