Innate immune response of Clara cells and alveolar macrophages induced by LPS prevent asthma development

GARCÍA LN , LEIMBGRUBER C, URIBE ECHEVARRIA EM MALDONADO C

Córdoba

Jornada; XIV Jornadas de Investigación Científica de la Facultad de Ciencias Médicas; 2013

Secretaría de Ciencia y Tecnología. Facultad de Ciencias Médicas, UNC

A possible reason for the increasing incidence of asthma is the lack of microbial stimulating signals of the modern lifestyle. It has been suggested that a response triggered by environmental endotoxins, such as the Escherichia Coli lipopolysacharide (LPS), through innate immune receptors like Toll- like receptors (TLR), may counterbalance the T helper (Th)-2 response of atopic asthma. Key component to lung innate immunity are Alveolar Macrophages (AM) and bronchiolar Clara Cells (CC), which secrete immunomodulatory proteins like CC16 and surfactant-D (SP-D) that modulate the allergic inflammation. However, both CC and AM, are affected by asthma. Accordingly, our aim was to examine if the pretreatment of CC and AM with LPS can prevent asthma development. We applied an asthma murine model developed in Balb/c adults female (n=12 per group) that were submitted to Ovoalbumin (OVA) (1mg/ml) intraperitoneal sensitization (day 0 and 14) followed by OVA (1mg/ml) daily intranasal challenges (day 24-33). A second group, LPSOVA, received intranasal LPS stimulus (10μg/50μl) previous (day -3 and -1) to OVA. ANOVA-Tukey test (p