INICSA   23916
INSTITUTO DE INVESTIGACIONES EN CIENCIAS DE LA SALUD
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
The anti-mitogenic effect of TGFb1 in pituitary tumor cells is enhanced by the inhibition of PI3K/Akt and MEK/ERK1/2 pathways
Autor/es:
PETITI JUAN PABLO; SABATINO MARÍA EUGENIA; GUTIÉRREZ SILVINA; SOSA LILIANA DEL VALLE; VACA ALICIA MALDRE; DE PAUL ANA LUCÍA; TORRES ALICIA INÉS
Lugar:
Bariloche
Reunión:
Simposio; Second South American Spring Symposium in Signal Transduction and Molecular Medicine; 2012
Resumen:
TGFb1 is a tumor suppressor that induces cell cycle arrest through the activation of Smads, which in turn can be regulated by other signal pathways. Our objective was to determine the contribution of the PI3K/Akt and MEK/ERK1/2 pathways as modulators of TGFb1-induced anti-proliferative response in tumoral adenohypophysial cells. GH3B6 cells were treated with TGFb1 (4ng/ml, 30 min or 24h) in presence or absence of the inhibitors: LY294002 (PI3K 10uM) and PD98059 (MEK1/2; 50uM). Cell cycle was analyzed by flow cytometry and the PRL secretion by RIA. The interaction of Smad2/3 with Akt or ERK1/2 was determined by immunoprecipitation and subsequent Western blot. The translocation of Smad2/3 and ERK1/3 was observed by confocal microscopy. Statistics: ANOVA-Fisher. The TGFb1 treatment induced Smad2/3 phosphorylation and cell arrest in G0/G1 phase (p