INICSA   23916
INSTITUTO DE INVESTIGACIONES EN CIENCIAS DE LA SALUD
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
MELATONIN REVERSES THE INHIBITORY EFFECT OF MENADIONE ON INTESTINAL CALCIUM ABSORPTION
Autor/es:
ARECO V, CARPENTIERI A, MARCHIONATTI A, PEREZ A, CENTENO V, TOLOSA DE TALAMONI N,
Lugar:
Buenos Aires
Reunión:
Workshop; Frontiers in BioScience; 2012
Institución organizadora:
Max Planck Society and the Ministry of Science, Technology and Innovation
Resumen:
Melatonin (MEL) is a hormone that, in addition to the circadian system regulation, is also attributed to have antioxidant properties. In our laboratory we have observed that MEL reverses the inhibitory effect of menadione (MEN) on intestinal calcium absorption (Ca2+). The aim of this study was to elucidate the molecular mechanisms that mediate the protective effect of MEL in the gut against the oxidant MEN. For this purpose, 4 week old chicks, were divided into four groups: 1) control, 2) treated i.p. with MEN (2.5 µmol / Kg of b.w.), 3) treated i.p. with MEL (10 mg /Kg of b.w.) and 4) treated with MEL i.p. after administration i.p. MEN.  Glutathione (GSH) content and the enzyme activities of antioxidant system were measured by spectrophotometric techniques. Apoptosis was analyzed by TUNEL technique and the caspase-3 activity. The results show that the decrease of GSH content and the increased activity of superoxide dismutase and catalase caused by MEN, returned to control values ​​after MEL treatment. Apoptosis triggered by MEN in intestinal cells was blocked by MEL, as indicated by the normalization of caspase-3 activity and DNA fragmentation. In conclusion, MEL reverses the inhibition of intestinal Ca 2+ absorption produced by MEN counteracting the oxidative stress and apoptosis. These findings suggest that MEL could be a potential drug of choice for the reversal of impaired intestinal Ca 2+ absorption produced by oxidative stress.