Application of inter-simple sequence repeat markers in the analysis of populations of the Chagas disease vector Triatoma infestans (Hemiptera, Reduviidae)

MARÍA F. RESTELLI; BEATRIZ A. GARCÍA; ALICIA R. PÉREZ DE ROSAS; MARÍA J. BLARIZA; CINTIA J. FERNÁNDEZ

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE

AMER SOC TROP MED & HYGIENE

Año: 2017 vol. 96 p. 660 - 663

0002-9637

Here we apply inter-simple sequence repeat (ISSR) markers to explore the fine-scale genetic structureand dispersal in populations of Triatoma infestans. Five selected primers from 30 primers were used to amplify ISSRsby polymerase chain reaction. A total of 90 polymorphic bands were detected across 134 individuals captured from11 peridomestic sites from the locality of San Martín (Capayán Department, Catamarca Province, Argentina). Significantlevels of genetic differentiation suggest limited gene flow among sampling sites. Spatial autocorrelation analysisconfirms that dispersal occurs on the scale of ∼469 m, suggesting that insecticide spraying should be extended atleast within a radius of ∼500 m around the infested area. Moreover, Bayesian clustering algorithms indicated geneticexchange among different sites analyzed, supporting the hypothesis of an important role of peridomestic structuresin the process of reinfestation.