Naringin prevents the inhibition of intestinal Ca 2+ absorption induced by a fructose rich diet

TOLOSA DE TALAMONI, N.; RODRÍGUEZ, V.; GUIZZARDI, S.; RIVOIRA, M.

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

ELSEVIER SCIENCE INC

Año: 2017 vol. 636 p. 1 - 10

0003-9861

This study tries to elucidate the mechanisms by which fructose rich diets (FRD) inhibit the rat intestinal Ca2+absorption, and determine if any or all underlying alterations are prevented by naringin (NAR). Male rats weredivided into: 1) controls, 2) treated with FRD, 3) treated with FRD and NAR. The intestinal Ca2+ absorption andproteins of the transcellular and paracellular Ca2+ pathways were measured. Oxidative/nitrosative stress andinflammation parameters were evaluated. FRD rats showed inhibition of the intestinal Ca2+ absorption anddecrease in the protein expression of molecules of both Ca2+ pathways, which were blocked by NAR. FRD ratsshowed an increase in the superoxide anion, a decrease in the glutathione and in the enzymatic activities of theantioxidant system, as well as an increase in the NO content and in the nitrotyrosine content of proteins. Theyalso exhibited an increase in both IL-6 and nuclear NF-κB. All these changes were prevented by NAR. In conclusion,FRD inhibit both pathways of the intestinal Ca2+ absorption due to the oxidative/nitrosative stress andinflammation. Since NAR prevents the oxidative/nitrosative stress and inflammation, it might be a drug to avoidalteration in the intestinal Ca2+ absorption caused by FRD