INSTITUTO DE INVESTIGACIONES EN CIENCIAS DE LA SALUD
Unidad Ejecutora - UE
The effects of trans-fatty acids on TAG regulation in mice depend on dietary 2 unsaturated fatty acids
SAIN J; SCALERANDI MV; LAVANDERA JV; GONZÁLEZ MA; SAIN J; BERNAL CA; SCALERANDI MV; GONZÁLEZ MA; BERNAL CA; LAVANDERA JV
BRITISH JOURNAL OF NUTRITION
CAMBRIDGE UNIV PRESS
Lugar: Cambridge; Año: 2016 vol. 116 p. 611 - 611
The aim of this study was to investigate the effects of trans fatty acids (TFA) on liver and serum triacylglycerol (TAG) regulation in mice fed diets containing different proportions of n 3, n 6 and n 9 unsaturated fatty acids (UFA) from olive (O), corn (C) or rapeseed (R) oils partially substituted or not with TFA (Ot, Ct and Rt, respectively). Male CF1 mice were fed (30 d) with one of these diets. The effects of the partial substitution (1%, w/w) of different UFA with TFA on the activity and expression of hepatic enzymes involved in lipogenesis and FA oxidation were evaluated as well as their transcription factors expressions. Some of the mechanisms involved in the serum TAG regulation, hepatic VLDL-TAG secretion rate and lipoprotein lipase (LPL) activity were assessed. In liver, TFA induced an increase in TAG content in the Ot and Rt groups and this effect was associated with an imbalance between lipogenesis and β-oxidation. In the Ot group exacerbated lipogenesis may be one of the mechanisms responsible for the liver steatosis induced by TFA, while in Rt it has been related to a decreased β-oxidation, compared to their respective controls. The enhanced hepatic VLDL-TAG secretion in the Ot and Rt groups was compensated with a differential removal of TAG by LPL enzyme in extrahepatic tissues leading to unchanged serum TAG levels. In brief, the effects of low levels of TFA on liver and serum TAG regulation in mice depend on the dietary proportions of n 3, n 6 and n 9 UFA.