INSTITUTO DE DIVERSIDAD Y ECOLOGIA ANIMAL
Unidad Ejecutora - UE
Effect of the entomopathogenic nematode-bacterial symbiont complex onMeloidogyne hapla and Nacobbus aberrans in short-term greenhouse trials
CACCIA, M.; DOUCET, M.; RONDAN DUEÑAS, J.; MARRO, NICOLÁS; LAX, P.
ELSEVIER SCI LTD
Lugar: Amsterdam; Año: 2018 vol. 114 p. 162 - 162
Meloidogyne hapla and Nacobbus aberrans are plant-parasitic nematodes that form galls in the roots of infectedplants and cause important economic losses. Entomopathogenic nematodes (EPNs) of the genera Steinernema andHeterorhabditis infect and kill insects via toxins produced by their symbiotic bacteria. EPNs have shown to havean antagonistic effect on different plant-parasitic nematode species in field and greenhouse trials. The aim of thepresent work was to evaluate, in tomato plants in greenhouse, the effect of the application of three ArgentineEPN isolates, their symbiotic bacteria and cell-free supernatants, on a population of M. hapla and two populationsof N. aberrans. Sixty days after inoculation, the number of galls and egg masses, the nematode reproductionfactor (RF) and plant biomass were calculated. With a few exceptions, biomass was not affected by the differenttreatments. None of the plant-parasitic nematode populations was reduced by infective juvenile inoculation ofthe different EPN isolates. Bacterial action differed among populations; M. hapla was the most susceptible one,with a significant reduction in the number of galls, egg masses and RF caused by the application of the threebacterial strains. The most significant effect was produced by the cell-free supernatants on nematode RF, withreductions of 62?90%, caused by bacterial metabolites. The different inoculation alternatives of the EPN-bacterialsymbiont complex tested in the present work (infective juveniles, bacteria and cell-free supernatant) arecompared for the first time for plant-parasitic nematode species