Histomorphologic and ultrastructural recovery of myopathy in rats treated with Low Level Laser Therapy

SERVETTO N.; DI PIETRO A.; CREMONEZZI D.; CAMPANA VR.; SIMES J.

LASERS IN MEDICAL SCIENCE

SPRINGER LONDON LTD

Lugar: Miami; Año: 2017 vol. 32 p. 841 - 849

0268-8921

The purpose of the present work was to study the effect of Low Level Laser Therapy (LLLT): Helium-Neon (He-Ne) and Gallium Arsenide (Ga-As) laser on the histomorphology of muscle and mitochondria in experimental myopathy in rats. Thirty Suquía strain female rats were distributed in groups: A) control (intact), B) injured, C) injured and treated with He-Ne laser, D) injured and treated with Ga-As laser, E) irradiated with He-Ne laser on the non-injured muscle, and F) irradiated with Ga-As laser on the non-injured muscle.Myopathy was induced by injecting 0.05 mg/rat/day of adrenaline in the left gastrocnemius muscle at the same point on 5 consecutive days, in groups B, C and D. LLLT was applied with 9.5 J.cm-2 daily for 7 consecutive days in groups C, D, E and F. The muscles were examined with optic and electronic microscopy. The inflammation was classified as: absent, mild and intense and the degree of mitochondrial alteration was graded I, II, III and IV. Categorical data were statistically analyzed by Chi-square and the Fisher-Irwin Bilateral test, setting significant difference at p