Brazilian Amaryllidaceae: a source of acetylcholinesterase inhibitory alkaloids

DE ANDRADE, J. P.; GIORDANI, R. B.; TORRAS-CLAVERIA, L.; PIGNI, N. B.; BERKOV, S.; KONRATH, E.; BUENO, K.; SACHETT, L. G.; DUTILH, J. H.; DE SOUZA BORGES, W.; VILADOMAT, F.; NAIR, J. J.; HENRIQUES, A. T.; ZUANAZZI, J. A. S.; BASTIDA, J.

Aguas de Lindoia

Congreso; 38a Reuniao Anual da Sociedade Brasileira de Química; 2015

Nine Brazilian Amaryllidaceae species were studied for their alkaloid composition and acetylcholinesterase (AChE) inhibitory activity via GC-MS and a modified Ellman assay, respectively. A total of thirty-six lkaloids were identified in these plants, of which Hippeastrum papilio and H. glaucescens exhibited the highest galanthamine content and the best IC50 values against AChE. Furthermore, H. vittatum and Rhodophiala bifida alsoshowed notable AChE inhibitory effects. X-ray crystallographic data for our galanthamine-type compounds revealed significant differences in theorientation of the N-methyl group, which are shown to be related to AChE inhibition.