Determination of differential metal and metalloids accumulation in gill, kidney, hepatopancreas and muscle and biomarkers response in Centropomus parallelus in neotropical estuaries

SOUZA, I.C.; MOROZESK, M.; SAKURAGUI, M; TAVARES, D; MONFERRÁN, M.V; WUNDERLIN D.A.

Edinburgh

Congreso; 11th International Congress on the Biology of Fish; 2014

11th International Congress on the Biology of Fish

We report levels of metals and metalloids (B, Al, V, Cr, Mn, Fe, Ni, Cu, Zn, As, Se, Ag, Cd, Hg and Pb) in muscle, hepatopancreas, kidney and gill tissues as well as their influence in biochemical biomarkers of exposure and effect in the native fish Centropomus parallelus from two neotropical estuaries affected by different anthropogenic factors. Metal analysis was performed with inductively-coupled plasma-optic emission spectroscopy (ICP-OES). Biomarkers measured were catalase, superoxide dismutase, glutathione-S-transferase, reduced glutathione, Na+/K+-ATPase, H+-ATPase, metallothionein, acetylcholinesterase, lipid hydroperoxides and oxidatively modified proteins. Multivariate statistics were used to indicate both spatial and temporal changes in metal composition of fish tissues and correlate with health status of inhabitant fish, showing the biggest levels of metal accumulation on gills and expressive biochemical alterations in Vitória Bay by reduced glutathione, Na+/K+-ATPase, H+-ATPase, metallothionein, acetylcholinesterase, lipid hydroperoxides and oxidatively modified proteins