ICYTAC   23898
INSTITUTO DE CIENCIA Y TECNOLOGIA DE ALIMENTOS CORDOBA
Unidad Ejecutora - UE
artículos
Título:
Sensitive biomarker responses of the shrimp Palaemonetes argentinusexposed to chlorpyrifos at environmental concentrations: Roles ofalpha-tocopherol and metallothioneinsLidwina
Autor/es:
LIDWINA BERTRAND; ROCIO BONANSEA; MONFERRAN M.V.; RAMON ASIS; CATHERINE MOUNEYRAC; MARIA VALERIA AME
Revista:
AQUATIC TOXICOLOGY
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2016 vol. 179 p. 72 - 81
ISSN:
0166-445X
Resumen:
The aim of this study was to evaluate the toxic effects of chlorpyrifos (CPF) at environmental con-centrations on the shrimp Palaemonetes argentinus, a South American native species. Organisms wereexposed to environmentally relevant concentrations of CPF (from 3.5 to 94.5 ng CPF L−1) at laboratory conditions for 96 h. A wide battery of biochemical responses including bioaccumulation, damage anddefense biomarkers were measured in cephalothorax and abdomen of shrimp.The concentration of CPF was below the detection limit of the method in both body sectors(8 ng CPF g−1ww), probably indicating fast biotransformation of the parental compound. Our resultsshowed that CPF exposure inhibits acetylcholinesterase activity from 3.5 ng CPF L−1, a concentrationbelow the suggested Argentinean guidelines for the protection of aquatic biota. Moreover, oxidative stresswas evidenced by increased H2O2content and increased levels of TBARs and carbonyl groups in proteins.The induction of antioxidant enzymes like catalase, glutathione S-transferase and glutathione peroxidaseseems not be sufficient to prevent oxidative damages. In addition, the mobilization of -tocopherol fromabdomen to cephalothorax was observed and reported for the first time in non-reproductive condition.Likewise, a strong diminution of metallothioneins occurred in cephalothorax from the lowest CPF concen-tration while induction occurred from the same treatment in abdomen as an oxidative stress response.Finally, significant correlation between Integrated Biomarker Response values and exposure concentra-tions suggest the usefulness of P. argentinus as bioindicator of CPF exposure at concentrations as low asenvironmental ones.