Fumigant toxicity of Citrus sinensis essential oil on Musca domestica L. adults in the absence and presence of a P450 inhibitor

YANINA ESTEFANÍA ROSSI, SARA MARÍA PALACIOS

ACTA TROPICA

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2013 vol. 127 p. 33 - 37

0001-706X

Essential oils (EOs) are potential tools for controlling Musca domestica L. In a fumigant assay, M. domes-tica adults treated with Citrus sinensis EO (LC50= 3.9 mg/dm3), with (4R)(+)-limonene (95.1%) being itsmain component, died within 15 min or less. The terpenes absorbed by the flies and their metabolites,analyzed using SPME fiber, were (4R)(+)-limonene (LC50= 6.2 mg/dm3), -pinene (LC50= 11.5 mg/dm3),-pinene (LC50= 6.4 mg/dm3), and two new components, carveol (LC50= 1122 mg/dm3) and carvone(LC50= 19 mg/dm3), in a proportion of 50, 6.2, 12.5, 6.3 and 25%, respectively. Carveol and carvone wereformed by oxidation of (4R)(+)-limonene mediated by cytochrome P450, as was suggested by a fumiga-tion assay on flies previously treated with piperonyl butoxide, a P450 inhibitor. In this experiment, anincrease in the toxicity of the EO and (4R)(+)-limonene was observed, as well as a lower production ofcarveol and carvone.