Canonical and non-canonical stimulation of T-ALL tumor cells and mesenchymal stromal cells determines tumoral niches with differential behavior.

AMOROS M ; CAYROL F.; GUTIÉRREZ L; BAYO JM; FARIAS R; CERCHIETTI L; GARCIA MG; LUZZANI C; MIRIUKA S; CORREA A; CREMASCHI GA; BOLONTRADE M

Congreso; Reunión Conjunta de Sociedades de Biociencias.; 2017

T-cell acute lymphoblastic leukemia (T-ALL) constitutes 20% of diagnosed ALL. As other hematological tumors it presents a close connection with the stromal counterpart. Leukemia cells can adjust the niche affecting the communication between the stroma and HSC in models of T-ALL. Thyroid hormone (TH) modulation may be critical in determining cancer progression. In this context we investigated the acquired functional behavior of mesenchymal stromal cells (MSC) when regulated by T-ALL cells in a TH-modulated microenvironment. We demonstrated that TH stimulation on T-ALL cells via surface receptors (non-canonical TH action) induced a higher chemotactic response and stronger morphogenic rearrangements, while canonical TH stimulation on tumor cells induced less chemotactic response (1.5 fold higher, 148±23 MSC/field, p