Influencia del sistema nervioso periférico sobre las actividades enzimáticas de 3-beta-HSD y 20-alfa-HSD en ovario en diferentes estados reproductivos en la rata

VEGA OROZCO A; VALLCANERAS S; DELGADO M; PELLARIN C; SOSA Z

Potrero de los Funes - San Luis.

Congreso; XXIV Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2006

Influence of the peripherical nervous system on the enzymatic activities of 3 b- HSD and 20 a-HSD in the ovary in different state reproductives of the rat.   Vega Orozco A, Vallcaneras S, Delgado M, Pellarin C, Sosa Z . Laboratorio de Biología de la Reproducción (LABIR). FQB y F. UNSL. San Luis. Argentina. amras@unsl.edu.ar Most of the fibers that constitutes the innervation of the ovary, make synapses in the coeliac ganglion (CG) and superior mesenteric ganglion (SMG). Objective: to elucidate the rol of the peripherical nervous system in enzymatic activity of the synthesis 3 b-HSD and degradation 20-a-HSD enzymes of the ovarian progesterone in different physiological conditions in the rat. Previous was standardized the integrated system CG-superior ovarian nerve-Ovary and SMG-ovarian nervous plexus-Ovary in rats. Student´s t-test was applied with a significance of p ≤ 0.05.1St Androstenedione (10-6M),was added in CG in pregnancy rats at 21 days. 2nd Acetylcholine (10-6M) was added in GMS in dioestrus days during oestrous cycle. 1st.The activity of 3 b- HSD increase while that 20 a- HSD decrease, both significantly (p ≤ 0.05) respects to the control groups.2nd. Dioestrus 1: 3 b- HSD diminished significantly versus the control group (p ≤ 0.01) while that 20 a-HSD increased with respects the control group (p ≤ 0.05). In dioestrus 2 day the activity of 3 b-HSD and 20 a- HSD do not showed significant changes with respects the group control. The results would indicate that the physiologic functioning of the corpora lutea is regulated do not only by the ovarian steroids only also by the influence of the neurotransmitters from to peripherical nervous system.