INVESTIGADORES
VALLCANERAS Sandra
congresos y reuniones científicas
Título:
Progesterona en ganglio celíaco actúa como un factor de sobrevida luteal al final de la preñez en la rata
Autor/es:
BURDISSO JUAN; VALLCANERAS SS; ANZULOVICH AC; CASAIS M; RASTRILLA AM
Lugar:
Potrero de los Funes ? San Luis.
Reunión:
Congreso; XXIV Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2006
Resumen:
PROGESTERONE IN COELIAC GANGLION ACTS AS A LUTEAL SURVIVAL FACTOR AT THE END OF RAT PREGNANCY Burdisso J, Vallcaneras S, Anzulovich AC, Casais M, Rastrilla AM. Fac Qca Bqca Fcia. Univ Nac San Luis, 5700 San Luis. In rat, the corpus luteum suffers regression at the end of pregnancy. Progesterone (Pg) is a luteal survival factor, and LH at the end of gestation is luteolitic. Our purpose was to investigate the participation of coeliac ganglion in the Pg luteotrophic action and LH influence. Materials and Methods: the ex vivo coeliac ganglion-superior ovarian nerve-ovary system (CG-SON-O) of 21 days of pregnancy was used. The system was incubated in 2 ml Krebs- Ringer buffer bicarbonate pH 7.4 (glucose and albumin 0.1 mg/ ml) at 37ºC for 240 min, with a 30-min preincubation. The groups were: 1- control, 2- PgG (addition of Pg 10-5M in CG), 3- LHo (ovine LH 50 ng/ml in ovary), 4- PgG-LHo (Pg 10-5M in CG and ovine LH 50 ng/ml in ovary). The hormones were added at time 0 (after preincubation). Pg by RIA (30, 60, 120, 180 and 240 min) was determined in the ovarian incubation liquid, and DNA fragmentation was analyzed by ladder in the corpora lutea at 240 min. Statistics: ANOVA-Duncan test. Results: a- Pg liberation in ovarian incubation liquid: PgG group vs control group: Pg significantly increased depending on time (*p<0.01). LHo group vs control group: no significant differences were observed. PgG-LHo group vs LH group: Pg significantly increased after 60 min (*p<0.01) and at 240 min, it reached the PgG group values (*p<0.01). b- DNA laddering decreased in those groups in which PgG was added in the ganglionic compartment. Conclussion: Pg from CG via SON would rescue the corpus luteum from regression without significant LH influence.