INVESTIGADORES
VALLCANERAS Sandra
congresos y reuniones científicas
Título:
Progesterona en ganglio celíaco actúa como un factor de sobrevida luteal al final de la preñez en la rata
Autor/es:
BURDISSO JUAN; VALLCANERAS SS; ANZULOVICH AC; CASAIS M; RASTRILLA AM
Lugar:
Potrero de los Funes ? San Luis.
Reunión:
Congreso; XXIV Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2006
Resumen:
PROGESTERONE IN COELIAC GANGLION ACTS AS A
LUTEAL SURVIVAL FACTOR AT THE END OF RAT
PREGNANCY
Burdisso J, Vallcaneras S, Anzulovich AC, Casais M, Rastrilla AM.
Fac Qca Bqca Fcia. Univ Nac San Luis, 5700 San Luis.
In rat, the corpus luteum suffers regression at the end of pregnancy.
Progesterone (Pg) is a luteal survival factor, and LH at the end of
gestation is luteolitic. Our purpose was to investigate the participation
of coeliac ganglion in the Pg luteotrophic action and LH influence.
Materials and Methods: the ex vivo coeliac ganglion-superior
ovarian nerve-ovary system (CG-SON-O) of 21 days of
pregnancy was used. The system was incubated in 2 ml Krebs-
Ringer buffer bicarbonate pH 7.4 (glucose and albumin 0.1 mg/
ml) at 37ºC for 240 min, with a 30-min preincubation. The groups
were: 1- control, 2- PgG (addition of Pg 10-5M in CG), 3- LHo
(ovine LH 50 ng/ml in ovary), 4- PgG-LHo (Pg 10-5M in CG and
ovine LH 50 ng/ml in ovary). The hormones were added at time 0
(after preincubation). Pg by RIA (30, 60, 120, 180 and 240 min)
was determined in the ovarian incubation liquid, and DNA fragmentation
was analyzed by ladder in the corpora lutea at 240 min.
Statistics: ANOVA-Duncan test. Results: a- Pg liberation in ovarian
incubation liquid: PgG group vs control group: Pg significantly
increased depending on time (*p<0.01). LHo group vs control
group: no significant differences were observed. PgG-LHo group
vs LH group: Pg significantly increased after 60 min (*p<0.01)
and at 240 min, it reached the PgG group values (*p<0.01). b- DNA
laddering decreased in those groups in which PgG was added in
the ganglionic compartment. Conclussion: Pg from CG via SON
would rescue the corpus luteum from regression without significant
LH influence.