Androgenic ganglionic influence on ovarian physiology at the end of pregnancy rat

VALLCANERAS SANDRA; CASAIS MARILINA; DELGADO SILVIA; RASTRILLA ANA MARÍA

Villa de Merlo - San Luis

Congreso; XXII Reunión Anual de la Sociedad de Biología de Cuyo y II Reunión de la Sociedad Argentina de MicroscopÍa; 2004

Sociedad de Biología de Cuyo

   Androgenic ganglionic influence on ovarian physiology at the end of pregnancy rat     Vallcaneras S, Casais M, Delgado S, Rastrilla AM Lab. Biol. de la Reprod. (Labir) FQByF-UNSL. Email: mcasais@unsl.edu.ar Previously, we have shown that androstenedione (A2) in coeliac ganglion modifies the ovarian progesterone release (P) from the coeliac ganglion-superior ovarian nerve-ovary (CG-SON-O) system of rats at the end of pregnancy. In the present work we study, in that system, whether the A2 effect is mediated by androgen receptors of CG and also, we analyze its relation with the nitric oxide release (NO) from the ovary. The system CG-SON-O was incubated in Krebs Ringer-glucose-albumin (0.1mg/ml) at 37º, keeping CG and O connected by the SON, in separate cuvettes. The CG was stimulated with A2 10-6 M (A2)g, Flutamide 10-4M (Flu)g (an antagonist of androgen receptor) and both (A2+Flu)g, respectively, to measure at 30, 60 and 120 min P (by RIA) and NO (Griess reactive). Controls without stimulation. Student?s test and Anova were used. Significance p<0.05. The results was: Progesterone ng/mg±SEM ovary (control= 30?: 0.032±0.003; 60?: 0.042±0.005; 120?: 0.039±0.008), with (A2)g and (Flu)g the P release was increased at all times in relation to control (p<0.01) and (p<0.05) respectively. In addition, the (A2)g effect on P release was higher than those of (Flu)g and (A2+Flu)g (p<0.05). NO nmoles/mg± SEM ovary (control= 30?: 2.54±0.77; 60?: 2.±0.5; 120?: 1.98±0.5), the (A2)g increased the NO release compared with control, (Flu)g and (A2+Flu)g (p<0.01). The results suggest that CG androgen receptors participate in the ovarian physiologic response.