INVESTIGADORES
VALLCANERAS Sandra
congresos y reuniones científicas
Título:
Estradiol stimulates ovaric GnRH liberation through a direct and indirect effect from the CG
Autor/es:
BRONZI D,; DANERI C; VALLCANERAS S; CASAIS M; SOSA Z.; RASTRILLA
Reunión:
Congreso; XXX Reunión Anual de la Sociedad de Biología de Cuyo; 2012
Resumen:
ESTRADIOL STIMULATES OVARIC GnRH LIBERATION THROUGH A DIRECT AND INDIRECT EFFECT FROM THE CG. Bronzi D, Daneri C, Valcaneras S, Casais M,  Sosa Z. Rastrilla AM. LABIR. U.N.S.L. zsosa@unsl.edu.ar   Estradiol (E2) is involved in the regulation of reproductive processes, acting on peripheral organs and sympathetic neurons associated with reproduction. The objective was to evaluate if E2 added in coeliac ganglion (CG) and in ovary alone modulates the ovarian release of GnRH and steroidogenic hormones in DII. We used two experimental schemes: a)- the CG-superior ovarian nerve (SON)-ovary system with and without the presence of E2 (10-8M) in ganglion compartment and b)- incubations of ovary alone with and without the presence of E2 (10-8M). The systems in both case were incubated in Krebs Ringer at 37ºC, in specific cuvettes designed for this purpose. Periodic extractions of the ovarian incubation liquid were carried out at 60 and 120 min. A2 , E2 and  GnRH and were measured by RIA. ANOVA 1 followed by Tuckey test with a statistical significance of p<0.05 was used. In CG-SON-ovary system, E2 in CG increased ovaric GnRH (120 min : 2.49±0.1 vs. 4.50±0.3), E2 (60min 2.6±0.2 vs 10.51± 2.4; 120 min 924.5± 90.3vs 2812± 540)(p<0.001). In incubations of ovary alone, E2 increased GnRH (120 min.: 4.1±0.9 vs 12.4±1.2) while A2 in both systems not present significant changes. We conclude that through an indirect effect on the ovary E2 favors luteal survival and the liberation of GnRH, in association with neural modulation from the CG via the SON.