COLONIES INITIALIZATION UNDER LABORATORY CONDITIONS OF NYSSOMYIA NEIVAI AND MIGONEMYIA MIGONEI (PSYCHODIDAE: PHLEBOTOMINAE) IN THE NORTH OF SALTA PROVINCE, ARGENTINA.

COPA, GN; ALMAZAN, MC; ESCALADA, A; ARAMAYO, LV; CIMINO, RO; NASSER, JR; GIL, JF

Mar del Plata

Encuentro; XXXI Annual Meeting of Sociedad Argentina de Protozoología (SAP); 2019

Sociedad Argentina de Protozoologia

Leishmaniases are vector-borne diseases with sand fly insects(Psychodidae: Phlebotominae) as vectors. In Oran (Salta, Argentina) Nyssomyianeivai is the most prevalent species followed by Migonemyia migonei, they havemedical relevance because were found infected with Leishmania sp. in Argentinaand Brazil. The establishment and maintenance of sand flies in colonies resultskey to study their biology, behavior, and relationships with pathogens. The aimof our work was to study the life cycle of Ny. neivai and Mg. migonei under laboratoryconditions and to elaborate a horizontal life table for them. For this, sandflies were captured in a peridomiciliary área of Orán city. The blood fedfemales were captured using manual aspirators both on domestic animals and treebark. A female with 5 males were maintained in rearing pots at 25 ± 2 ° C and85-95 % relative humidity. Larval food consisted of a mixture of rabbit feces,fish feed, rabbit feed, while adult sand fly food provided was sugar solution(30 %). A total of 82 females were conditioned for oviposition, the 41.4 % ofthem survived and oviposited. Thirty-two specimens were Ny. neivai and two Mg. migonei.The average number of eggs laid per female were 40.81 (Ny. neivai) and 59.50(Mg. migonei). A total of 78 adults of Ny. neivai and 27 of Mg. migonei(p<0.001) emerged under laboratory conditions. For Ny. neivai and Mg.migonei, the time range occurred between the egg and adult stages was 37 and 36days, respectively. The proportions of the original surviving cohorts (lx) ineach stage, for both species, were higher in the first stage (L1). Theproportion of deaths per stage (dx) for Ny. neivai was higher in eggs and L1,while in Mg. migonei was in L2. Following this protocol, sand fly coloniescould be initiated under laboratory conditions, which will allow thedevelopment of future projects for incriminating vectors and reservoirs in thenorth of Argentina.